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WB
Western blot analysis of GAD65/GAD67 in mouse brain , rat brain lysates and using GAD65/GAD67 Goat pAb.IF
Immunofluorescence analysis of GAD65/GAD67(Green) in mouse brain tissue using GAD65/GAD67 Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded human prostate cancer tissue sections using the antibody GAD65/GAD67 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Product Name | GAD65/GAD67 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human GAD65/GAD67 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | NEB |
|---|---|
| Gene Synonyms | AMC6 NEM2 NEB177D |
| Gene Full Name | nebulin |
| Gene Summary | This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein. The encoded protein contains approximately 30-amino acid long modules that can be classified into 7 types and other repeated modules. Protein isoform sizes vary from 600 to 800 kD due to alternative splicing that is tissue-, species-,and developmental stage-specific. Of the 183 exons in the nebulin gene, at least 43 are alternatively spliced, although exons 143 and 144 are not found in the same transcript. Of the several thousand transcript variants predicted for nebulin, the RefSeq Project has decided to create three representative RefSeq records. Mutations in this gene are associated with recessive nemaline myopathy. [provided by RefSeq, Sep 2009] |
| Molecular Weight(MW) | 65 kDa |
| Function | GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form. |
WB
Western blot analysis of GAD65/GAD67 in mouse brain , rat brain lysates and using GAD65/GAD67 Goat pAb.
IF
Immunofluorescence analysis of GAD65/GAD67(Green) in mouse brain tissue using GAD65/GAD67 Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded human prostate cancer tissue sections using the antibody GAD65/GAD67 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
