WB
Western blot analysis of VGAT in mouse brain , rat brain lysates and using VGAT Goat pAb.IF
Immunofluorescence analysis of VGAT(Green) in mouse brain tissue using VGAT Goat pAb, and DAPI(Blue)| Product Name | SLC32A1 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human VGAT |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | SLC32A1 |
|---|---|
| Gene Synonyms | VGAT VIAAT DEE114 GEFSP12 VIAAT GEFSP12 |
| Gene Full Name | solute carrier family 32 member 1 |
| Gene Summary | The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 57 kDa |
| Function | The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005). |
| Cellular Localization | Cell projection,Cytoplasmic vesicle,Membrane,Synapse |
WB
Western blot analysis of VGAT in mouse brain , rat brain lysates and using VGAT Goat pAb.
IF
Immunofluorescence analysis of VGAT(Green) in mouse brain tissue using VGAT Goat pAb, and DAPI(Blue)| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
