WB
Sample:heart (Mouse) Lysate at 40 ug. Primary: Anti-SLC32A1 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution.IHC
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB(C-0010) staining.| Product Name | SLC32A1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human SLC30A9 : 351-450/525. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC32A1 |
|---|---|
| Gene Synonyms | VGAT VIAAT DEE114 GEFSP12 VIAAT GEFSP12 |
| Gene Full Name | solute carrier family 32 member 1 |
| Gene Summary | The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008] |
| Alternative Names | DEE114 GEFSP12 VGAT VIAAT VIAAT GEFSP12 RUNC-47 VIAAT_HUMAN SLC32A1 GABA and glycine transporter Solute carrier family 32 member 1 Vesicular GABA transporter (hVIAAT) VIAAT_MOUSE Vesicular GABA and glycine transporter Vesicular GABA transporter (mVGAT | mVIAAT) VIAAT_RAT Vesicular GABA transporter (rGVAT) rat UNC-47 homolog (RUNC-47) vesicular inhibitory amino acid transporter solute carrier family 32 (GABA vesicular transporter) member 1 |
| Molecular Weight(MW) | 57 kDa(Observed band size:60 kDa) |
| Cellular Localization | Cytoplasmic vesicle membrane; Multi-pass membrane protein |
WB
Sample:heart (Mouse) Lysate at 40 ug. Primary: Anti-SLC32A1 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution.
IHC
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB(C-0010) staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
