WB
Western blot analysis of Claudin 5 in mouse colon , rat colon lysates and using Claudin 5 Goat pAb.IF
Immunofluorescence analysis of Claudin 5(Green) in mouse colon tissue using Claudin 5 Goat pAb, and DAPI(Blue)| Product Name | Claudin 5 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Claudin 5 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | CLDN5 |
|---|---|
| Gene Synonyms | AWAL BEC1 TMVCF TMDVCF CPETRL1 |
| Gene Full Name | claudin 5 |
| Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018] |
| Molecular Weight(MW) | 21 kDa |
| Function | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found. |
| Cellular Localization | Cell junction,Cell membrane,Membrane,Tight junction |
WB
Western blot analysis of Claudin 5 in mouse colon , rat colon lysates and using Claudin 5 Goat pAb.
IF
Immunofluorescence analysis of Claudin 5(Green) in mouse colon tissue using Claudin 5 Goat pAb, and DAPI(Blue)| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
