WB
Western blot analysis of Claudin 5 on different lysates with Rabbit anti-Claudin 5 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Claudin 5 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Claudin 5 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Claudin 5 aa 191-214 / 218. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CLDN5 |
|---|---|
| Gene Synonyms | AWAL BEC1 TMVCF TMDVCF CPETRL1 |
| Gene Full Name | claudin 5 |
| Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018] |
| Alternative Names | Androgen withdrawal and apoptosis induced protein RVP1 like antibody AWAL antibody BEC 1 antibody BEC1 antibody Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody Claudin-5 antibody Claudin5 antibody CLD5_HUMAN antibody CLDN 5 antibody Cldn5 antibody Androgen withdrawal and apoptosis induced protein RVP1 like antibody AWAL antibody BEC 1 antibody BEC1 antibody Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody Claudin-5 antibody Claudin5 antibody CLD5_HUMAN antibody CLDN 5 antibody Cldn5 antibody CPETR L1 antibody CPETRL 1 antibody CPETRL1 antibody TMDVCF antibody TMVCF antibody Transmembrane protein deleted in VCFS antibody Transmembrane protein deleted in velocardiofacial syndrome antibody |
| Molecular Weight(MW) | 23 kDa(Observed band size: 20 kDa) |
| Cellular Localization | Cell junction. Cell membrane. |
WB
Western blot analysis of Claudin 5 on different lysates with Rabbit anti-Claudin 5 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Claudin 5 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 1075. |
