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WB
Western blot analysis of α-Synuclein in mouse brain , rat brain lysates and using α-Synuclein Goat pAb.IF
Immunofluorescence analysis of α-Synuclein(Green) in mouse brain tissue using α-Synuclein Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded human skin tissue sections using the antibody α-Synuclein Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | alpha Synuclein Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human α-Synuclein |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | SNCA |
|---|---|
| Gene Synonyms | PD1 NACP PARK1 PARK4 |
| Gene Full Name | synuclein alpha |
| Gene Summary | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016] |
| Molecular Weight(MW) | 18 kDa(Observed band size: 15 kDa) |
| Function | May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). |
| Cellular Localization | Amyloid,Cell projection,Cytoplasm,Membrane,Nucleus,Secreted,Synapse |
WB
Western blot analysis of α-Synuclein in mouse brain , rat brain lysates and using α-Synuclein Goat pAb.
IF
Immunofluorescence analysis of α-Synuclein(Green) in mouse brain tissue using α-Synuclein Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded human skin tissue sections using the antibody α-Synuclein Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
