ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-Synuclein alpha(Tyr136) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA (OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-Synuclein alpha (Tyr136) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Tyr136 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000
|
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNCA |
|---|---|
| Gene Synonyms | PD1 NACP PARK1 PARK4 |
| Gene Full Name | synuclein alpha |
| Gene Summary | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016] |
| Alternative Names | Alpha synuclein Alpha-synuclein Alpha-synuclein isoform NACP140 alphaSYN MGC105443 MGC110988 MGC127560 MGC64356 NACP Non A beta component of AD amyloid Non A4 component of amyloid Non A4 component of amyloid precursor Non-A beta component of AD amyloid Non-A-beta component of alzheimers disease amyloid precursor of Non-A4 component of amyloid precursor Non-A4 component of amyloid precursor of OTTHUMP00000218549 OTTHUMP00000218551 OTTHUMP00000218552 OTTHUMP00000218553 OTTHUMP00000218554 PARK 1 PARK 4 PARK1 PARK4 Parkinson disease (autosomal dominant Lewy body) 4 Parkinson disease familial 1 SNCA Snca synuclein alpha (non A4 component of amyloid precursor) SYN Synuclein alpha Synuclein alpha 140 Synuclein alpha (non A4 component of amyloid precursor) SYUA_HUMAN |
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Amyloid, Cell projection, Cytoplasm, Membrane, Nucleus, Secreted, Synapse. |
| SwissProt ID | P37840 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-Synuclein alpha(Tyr136) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA (OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000
|
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
