WB
Western Blot,Sample: Rat Kidney lysate Primary Ab: 1.5µg/ml Mouse Anti-Human COL4a3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody| Product Name | Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | RPC143Hu01-Recombinant Collagen Type IV Alpha 3 (COL4a3) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 Kappa |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: 0.01-2µg/mL |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | COL4A3 |
|---|---|
| Gene Synonyms | ATS2,ATS3,BFH2,ATS3A,ATS3B |
| Gene Full Name | collagen type IV alpha 3 chain |
| Gene Summary | Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010] |
| Alternative Names | COL4-A3 Tumstatin Goodpasture Antigen Collagen Alpha-3(IV)chain |
WB
Western Blot,Sample: Rat Kidney lysate Primary Ab: 1.5µg/ml Mouse Anti-Human COL4a3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody| Application Notes | WB: 0.01-2µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
