Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Live| Product Name | Rabbit anti-COL4A3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-COL4A3 antibody: synthetic peptide directed towards the N terminal of human COL4A3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HorseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL4A3 |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | Collagen, type IV, alpha 3 (Goodpasture antigen) |
| Gene Summary | Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. There are multiple alternate transcripts that appear to be unique to this human alpha 3 gene and alternate splicing is restricted to the six exons that encode this C-terminal domain. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Some exons of this gene are interspersed with exons of an uncharacterized gene which is on the opposite strand. |
| Molecular Weight(MW) | 156kDa |
| Sequence | 1637 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM118287