WB
Western Blot,Samples: Lane1: Rat Mammary gland lysate,Lane2: Porcine Mammary gland lysate;Primary Ab: 0.2µg/ml Mouse Anti-Rat LEP AntibodySecond Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyWB
Western Blot,Samples: Lane1: MCF7 cell lysate,Lane2: T-47D cell lysate;Primary Ab: 0.2µg/ml Mouse Anti-Rat LEP AntibodySecond Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyWB
Western Blot,Sample: 3T3-L1 cell lysatePrimary Ab: 0.2µg/ml Mouse Anti-Rat LEP AntibodySecond Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody| Product Name | Monoclonal Antibody to Leptin (LEP) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | RPA084Ra01-Recombinant Leptin (LEP) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1Kappa |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: 0.01-2µg/mL |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | Lep |
|---|---|
| Gene Synonyms | ob,obese |
| Gene Full Name | leptin |
| Gene Summary | Enables DNA binding activity and hormone activity. Involved in several processes, including cell surface receptor signaling pathway,positive regulation of cytokine production,and positive regulation of signal transduction. Acts upstream of or within several processes, including determination of adult lifespan,regulation of protein localization,and steroid metabolic process. Located in cytoplasm and extracellular space. Is expressed in several structures, including adipose tissue,alimentary system,central nervous system,early conceptus,and reproductive system. Used to study abdominal obesity-metabolic syndrome (multiple),obesity,steatotic liver disease (multiple),and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence,congenital leptin deficiency,liver disease (multiple),lung disease (multiple),and type 2 diabetes mellitus. Orthologous to human LEP (leptin). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | OB OBS Obesity Homolog Obesity Factor Obese Protein |
WB
Western Blot,Samples: Lane1: Rat Mammary gland lysate,Lane2: Porcine Mammary gland lysate;Primary Ab: 0.2µg/ml Mouse Anti-Rat LEP AntibodySecond Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
WB
Western Blot,Samples: Lane1: MCF7 cell lysate,Lane2: T-47D cell lysate;Primary Ab: 0.2µg/ml Mouse Anti-Rat LEP AntibodySecond Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
WB
Western Blot,Sample: 3T3-L1 cell lysatePrimary Ab: 0.2µg/ml Mouse Anti-Rat LEP AntibodySecond Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody| Application Notes | WB: 0.01-2µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
