WB
Western blot analysis using CXCR5 antibody against JurKat(1), K562(2,)MCF-7(3),PC-12(4) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CXCR5 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCR5 |
|---|---|
| Gene Synonyms | BLR1 CD185 CXC-R5 CXCR-5 Gpcr6 MDR15 NLR |
| Gene Full Name | C-X-C motif chemokine receptor 5 |
| Gene Summary | This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| Alternative Names | Blr-1 burkitt lymphoma receptor 1 homolog Burkitt lymphoma receptor 1 GTP binding protein (chemokine (C-X-C motif) receptor 5) Burkitt lymphoma receptor 1 GTP-binding protein C Cmotif chemokine C X C motif chemokine CC motif chemokine CCmotif chemokine CD185 antigen chemochine (C-X-C motif) receptor 5 chemokine (C-X-C motif) receptor 5 CXC CXC motif chemokine CXC-R5 CXCR-5 Fusin GPR9 HUMSTR LESTR MDR-15 monocyte-derived receptor 15 neurolymphatic receptor |
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P32302 |
|---|
WB
Western blot analysis using CXCR5 antibody against JurKat(1), K562(2,)MCF-7(3),PC-12(4) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
