ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using Aurora B antibody against ,Mouse stomach tissue(1),Mouse liver tissue(2),Rat intestine tissue(3),Rat stomach tissue(4),Rat kidney tissue(5),Rat liver tissue(6) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Aurora B Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AURKB |
|---|---|
| Gene Synonyms | AIK AIK2 AIM-1 AIM1 AIRK1 AIRK2 ARK-1 ARK-2 ARK1 ARK2 AURA AurB aurkb-sv1 aurkb-sv2 Aurora-A Ayk1 BTAK IAK IAK1 IPL1 PPP1R47 PPP1R48 STK-1 STK12 STK15 STK5 STK6 STK7 |
| Gene Full Name | aurora kinase B |
| Gene Summary | This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | AIM-1 ARK-2 aurora 1 aurora 2 aurora A aurora B aurora family kinase 1 aurora kinase B-Sv1 aurora kinase B-Sv2 aurora- and IPL1-like midbody-associated protein 1 aurora- and Ipl1-like midbody-associated protein 1 homolog aurora-1 aurora-A aurora-B aurora-related kinase 1 aurora-related kinase 2 aurora/IPL1-like kinase aurora/IPL1-related kinase 1 aurora/IPL1-related kinase 2 breast tumor-amplified kinase IAK1 ipl1- and aurora-related kinase 1 protein kinase protein phosphatase 1 regulatory subunit 47 protein phosphatase 1 regulatory subunit 48 ratAurA serine/threonine kinase 12 serine/threonine kinase 5 serine/threonine kinase 6 serine/threonine protein kinase 15 serine/threonine-protein kinase 12 serine/threonine-protein kinase 15 serine/threonine-protein kinase 5 serine/threonine-protein kinase 6 serine/threonine-protein kinase aurora-A serine/threonine-protein kinase aurora-B serine/threonine-protein kinase Ayk1 STK6 aurora-A IPL1-related kinase AIK BTAK STK15 |
| Molecular Weight(MW) | 39kDa(observed:54kDa) |
| Cellular Localization | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus |
| SwissProt ID | Q96GD4 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using Aurora B antibody against ,Mouse stomach tissue(1),Mouse liver tissue(2),Rat intestine tissue(3),Rat stomach tissue(4),Rat kidney tissue(5),Rat liver tissue(6) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
