ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using Ctip1 antibody against Huvec(1),Cos-7(2),P19(3),C2C12(4),K562(5),Mfc(6) cell. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Ctip1 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL11A |
|---|---|
| Gene Synonyms | 2810047E18Rik BCL-11A CTIP1 D930021L15Rik DILOS EVI9 Evi9a Evi9b Evi9c HBFQTL5 mKIAA1809 SMARCM1 ZNF856 |
| Gene Full Name | BCL11 transcription factor A |
| Gene Summary | This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | B cell CLL/lymphoma 11A B cell CLL/lymphoma 11A (zinc finger protein) B-cell CLL/lymphoma 11A B-cell CLL/lymphoma 11A (zinc finger protein) B-cell lymphoma/leukemia 11A BAF chromatin remodeling complex subunit BCL11A BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) BCL11A BAF complex component BCL11A-XS zinc finger protein C2H2-type zinc finger protein COUP-TF interacting protein 1 COUP-TF-interacting protein 1 ecotropic viral integration site 9 homolog ecotropic viral integration site 9 protein ecotropic viral integration site 9 protein homolog EVI-9 FLJ10173 FLJ34997 myeloid leukemia zinc finger protein zinc finger protein 856 |
| Molecular Weight(MW) | 91kDa(observed:105kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
| SwissProt ID | Q9H165 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using Ctip1 antibody against Huvec(1),Cos-7(2),P19(3),C2C12(4),K562(5),Mfc(6) cell. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
