ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using KPNA2 antibody against Hela(1),Hepg2(2),K562(3),A549(4),Huvec(5),Cos-7(6),Hek293(7) cell,Mouse skin tissue(8) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | KPNA2 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KPNA2 |
|---|---|
| Gene Synonyms | 2410044B12Rik importin IPOA1 PTAC58 QIP2 RCH1 SRP1-alpha SRP1alpha |
| Gene Full Name | karyopherin subunit alpha 2 |
| Gene Summary | The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | IMA2 importin alpha 1 importin alpha 2 importin alpha P1 importin subunit alpha-2 importin-alpha-P1 IPOA 1 IPOA1 QIP2 karyopherin (importin) alpha 2 Karyopherin A2 karyopherin alpha 2 karyopherin alpha 2 (RAG cohort 1 importin alpha 1) m-importin-alpha-P1 nuclear import protein nuclear pore-targeting complex 58kD component pendulin pore targeting complex 58 kDa subunit RAG cohort 1 RAG cohort protein 1 RCH 1 SRP 1 SRP1 alpha SRP1-alpha |
| Molecular Weight(MW) | 58kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus |
| SwissProt ID | P52292 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using KPNA2 antibody against Hela(1),Hepg2(2),K562(3),A549(4),Huvec(5),Cos-7(6),Hek293(7) cell,Mouse skin tissue(8) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
