WB
Western blot analysis of KPNA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control:FC
Flow cytometric analysis of KPNA2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | KPNA2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human KPNA2 aa 41-90 / 529. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KPNA2 |
|---|---|
| Gene Synonyms | QIP2 RCH1 IPOA1 PTAC58 SRP1alpha SRP1-alpha |
| Gene Full Name | karyopherin subunit alpha 2 |
| Gene Summary | The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | IMA1_HUMAN antibody Importin alpha 1 antibody Importin alpha 2 antibody Importin alpha 2 subunit antibody Importin alpha P1 antibody Importin subunit alpha-1 antibody IPO A1 antibody IPOA 1 antibody IPOA1 antibody Karyopherin alpha 2 antibody Karyopherin alpha 2 RAG cohort 1 antibody Karyopherin alpha 2 subunit antibody Karyopherin subunit alpha-2 antibody KPNA2 antibody KPNA2 protein antibody Pendulin antibody QIP 2 antibody QIP2 antibody RAG cohort 1 antibody RAG cohort protein 1 antibody RCH 1 antibody RCH1 antibody SRP 1 antibody SRP1 alpha antibody SRP1 antibody SRP1-alpha antibody SRP1alpha antibody |
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus. |
WB
Western blot analysis of KPNA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control:
FC
Flow cytometric analysis of KPNA2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:2000 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
