WB
Various lysates were subjected to SDS PAGE followed by western blot with KPNA2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using KPNA2 antibody at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibodyat dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).| Product Name | KPNA2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human KPNA2 protein |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:300-1:1200 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | Human |
| Concentration | 1.7mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA2 |
|---|---|
| Gene Synonyms | QIP2 RCH1 IPOA1 PTAC58 SRP1alpha SRP1-alpha |
| Gene Full Name | karyopherin subunit alpha 2 |
| Gene Summary | The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | 2G9C1 Importin subunit alpha-1 IPO A1 IPOA 1 IPOA1 |
| Molecular Weight(MW) | 55-58kDa |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Golgi apparatus,Membrane,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with KPNA2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using KPNA2 antibody at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibodyat dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).| Application Notes | WB:1:1000-1:5000 IHC:1:300-1:1200 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
