WB
Western blot analysis using PIK3CD antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4)cell. 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Product Name | PIK3CD Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3CD |
|---|---|
| Gene Synonyms | 2410099E07Rik 2610208K16Rik APDS IMD14 IMD14A IMD14B p110D P110DELTA PI3K PI3Kdelta ROCHIS |
| Gene Full Name | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta |
| Gene Summary | Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.[provided by RefSeq, Jul 2010] |
| Alternative Names | catalytic phosphatidylinositol 3-kinase delta p100 delta phosphatidylinositol 3-kinase catalytic delta polypeptide phosphatidylinositol 3-kinase catalytic subunit delta phosphatidylinositol 3-kinase p110 delta phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta phosphatidylinositol-4 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta phosphatidylinositol-4 5-bisphosphate 3-kinase catalytic subunit delta short variant 8/20 phosphoinositide 3 kinase P110delta phosphoinositide-3-kinase C phosphoinositide-3-kinase catalytic delta polypeptide phosphoinositide-3-kinase catalytic delta polypeptide variant p37delta PI3-kinase p110 subunit delta PI3-kinase subunit delta PI3K delta PI3K-delta PI3K-p110delta pi3kcd PI3Kdelta PI3Kp110delta Pik3cd PIK3Kdelta PIK3Kdelta SV20 PIK3Kdelta SV8 PIK3Kdelta SV8/20 ptdIns-3-kinase subunit delta ptdIns-3-kinase subunit p110-delta |
| Molecular Weight(MW) | 119kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | O00329 |
|---|
WB
Western blot analysis using PIK3CD antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4)cell. 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
