WB
LNCaP cells were subjected to SDS PAGE followed by western blot with PIK3CD antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
Jurkat cells were subjected to SDS PAGE followed by western blot with PIK3CD antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | PI3 Kinase p110 Delta Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PIK3CD protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:400-1:600 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3CD |
|---|---|
| Gene Synonyms | APDS PI3K IMD14 p110D IMD14A IMD14B ROCHIS P110DELTA PI3Kdelta |
| Gene Full Name | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta |
| Gene Summary | Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.[provided by RefSeq, Jul 2010] |
| Alternative Names | p110D PI3 kinase subunit delta PI3K delta PIK3CD PtdIns 3 kinase subunit delta |
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Cytoplasm. |
WB
LNCaP cells were subjected to SDS PAGE followed by western blot with PIK3CD antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
Jurkat cells were subjected to SDS PAGE followed by western blot with PIK3CD antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:2000-1:10000 ICC/IF:1:400-1:600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
