WB
Figure 1: Western blot analysis using PIK3CG antibody against Hepg2(1) cell lysate. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-PIK3CG antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3CG |
|---|---|
| Gene Synonyms | 5830428L06Rik IMD97 p110gamma p120-PI3K PI3CG PI3K PI3Kgamma PIK3 |
| Gene Full Name | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma |
| Gene Summary | Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015] |
| Alternative Names | 1-phosphatidylinositol 3-kinase catalytic subunit p110-gamma phosphatidylinositol 3 kinase gamma p110 gamma phosphatidylinositol 3-kinase catalytic 110-kD gamma phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma phosphatidylinositol-4 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma phosphoinositide-3-kinase gamma catalytic subunit phosphoinositide-3-kinase catalytic gamma polypeptide PI3-kinase subunit gamma pi3kcg ptdIns-3-kinase subunit gamma ptdIns-3-kinase subunit p110-gamma serine/threonine protein kinase PIK3CG |
| Molecular Weight(MW) | 126kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Cytoskeleton, Membrane |
| SwissProt ID | P48736 |
|---|
WB
Figure 1: Western blot analysis using PIK3CG antibody against Hepg2(1) cell lysate. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
