WB
Western blot analysis using KDM5B antibody against A549(1) cell lysate. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-KDM5B antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KDM5B |
|---|---|
| Gene Synonyms | 2010009J12Rik 2210016I17Rik CT31 D1Ertd202e JARID1B mKIAA4034 MRT65 PLU-1 PLU1 PPP1R98 PUT1 Rb-Bp2 RBBP2H1A RBP2-H1 RGD1565602 |
| Gene Full Name | lysine demethylase 5B |
| Gene Summary | This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016] |
| Alternative Names | [histone H3]-trimethyl-L-lysine(4) demethylase 5B cancer/testis antigen 31 histone demethylase JARID1B HRIHFB2060 jumonji AT rich interactive domain 1B jumonji AT rich interactive domain 1B (Rbp2 like) Jumonji AT rich interactive domain 1B (RBP2-like) jumonji/ARID domain-containing protein 1B lysine (K)-specific demethylase 5B protein phosphatase 1 regulatory subunit 98 putative DNA/chromatin binding motif putative DNA/chromatin binding motif 1 retinoblastoma-binding protein 2 homolog 1 retinoblastoma-binding protein 2 homolog 1A |
| Molecular Weight(MW) | 176kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q9UGL1 |
|---|
WB
Western blot analysis using KDM5B antibody against A549(1) cell lysate. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
