WB
Western blot analysis using Ctip2 antibody against Hela(1),T24(2) cell. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-Ctip2 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL11B |
|---|---|
| Gene Synonyms | 9130430L19Rik ATL1 ATL1-alpha ATL1-beta ATL1-delta ATL1-gamma B630002E05Rik BCL-11B CTIP-2 CTIP2 hRIT1-alpha IDDFSTA IMD49 RIT1 SMARCM2 ZNF856B |
| Gene Full Name | BCL11 transcription factor B |
| Gene Summary | This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2013] |
| Alternative Names | B cell CLL/lymphoma 11B B-cell CLL/lymphoma 11B B-cell CLL/lymphoma 11B (zinc finger protein) B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein B-cell lymphoma/leukaemia 11B BAF chromatin remodeling complex subunit BCL11B BCL-11B BCL11B BAF complex component BCL11B/TRDC fusion COUP-TF interacting protein 2 COUP-TF-interacting protein 2 hRit1 mRit1 radiation-induced tumor suppressor gene 1 protein zinc f zinc finger protein hRit1 alpha |
| Molecular Weight(MW) | 96kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q9C0K0 |
|---|
WB
Western blot analysis using Ctip2 antibody against Hela(1),T24(2) cell. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
