WB
Western blot analysis of Ctip2 on different lysates with Rabbit anti-Ctip2 antibody at 1/500 dilution. Lane 1: LO2 cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Ctip2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of Ctip2 in LO2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).IF-P
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Ctip2 with Rabbit anti-Ctip2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IF-F
Immunofluorescence analysis of frozen mouse P0 brain tissue with Rabbit anti-Ctip2 antibody at 1/2,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/2,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Ctip2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Ctip2 aa 491-534 / 894. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| WB:1:1000 IHC:1:200-1:1000 ICC:1:50-1:200 IF-P:1:200-1:500 IF-F:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL11B |
|---|---|
| Gene Synonyms | ATL1 RIT1 CTIP2 IMD49 CTIP-2 IDDFSTA SMARCM2 ZNF856B ATL1-beta ATL1-alpha ATL1-delta ATL1-gamma hRIT1-alpha |
| Gene Full Name | BCL11 transcription factor B |
| Gene Summary | This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2013] |
| Alternative Names | ATL1 alpha antibody ATL1 antibody ATL1 beta antibody ATL1 gamma antibody ATL1-delta antibody B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody B cell lymphoma/leukemia 11B antibody B-cell CLL/lymphoma 11B (zinc finger protein) antibody B-cell CLL/lymphoma 11B antibody B-cell lymphoma/leukemia 11B antibody ATL1 alpha antibody ATL1 antibody ATL1 beta antibody ATL1 gamma antibody ATL1-delta antibody B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody B cell lymphoma/leukemia 11B antibody B-cell CLL/lymphoma 11B (zinc finger protein) antibody B-cell CLL/lymphoma 11B antibody B-cell lymphoma/leukemia 11B antibody BC11B_HUMAN antibody BCL-11B antibody Bcl11b antibody BCL11B/TRDC fusion antibody COUP TF interacting protein 2 antibody COUP-TF interacting protein 2 antibody COUP-TF-interacting protein 2 antibody Ctip 2 antibody CTIP-2 antibody CTIP2 antibody hRIT1 alpha antibody hRit1 antibody Radiation induced tumor suppressor gene 1 antibody Radiation induced tumor suppressor gene 1 protein antibody Radiation-induced tumor suppressor gene 1 protein antibody Rit 1 antibody Rit1 antibody zinc finger protein hRit1 alpha antibody ZNF856B antibody |
| Molecular Weight(MW) | 96kDa(Observed band size: 130kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of Ctip2 on different lysates with Rabbit anti-Ctip2 antibody at 1/500 dilution. Lane 1: LO2 cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Ctip2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of Ctip2 in LO2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
IF-P
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Ctip2 with Rabbit anti-Ctip2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-F
Immunofluorescence analysis of frozen mouse P0 brain tissue with Rabbit anti-Ctip2 antibody at 1/2,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/2,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC:1:50-1:200 IF-P:1:200-1:500 IF-F:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
