WB
Western blot analysis using Nav1.6/SCN8A antibody against Hela(1) ,A549(2),A431(3),Huvec(4),Nih3t3(5),Cos-7(6)cell . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-Nav1.6/SCN8A antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCN8A |
|---|---|
| Gene Synonyms | BFIS5 C630029C19Rik CERIII CIAT DEE13 dmu EIEE13 MED mnd-2 mnd2 MYOCL2 NaCh6 Nav1.6 nmf2 nmf335 nmf58 nur14 PN4 seal |
| Gene Full Name | sodium voltage-gated channel alpha subunit 8 |
| Gene Summary | This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with cognitive disability, pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| Alternative Names | ataxia 3 hNa6/Scn8a voltage-gated sodium channel Na+ channel naCh6 NaCh6 Scn8a peripheral nerve protein type 4 PN4 SCN8A Scn8a (PN4) sodium channel 6 sodium channel protein type VIII subunit alpha sodium channel voltage-gated type VIII alpha sodium channel voltage gated type VIII alpha subunit sodium channel voltage-gated type 8 alpha polypeptide sodium channel voltage-gated type 8 alpha subunit sodium channel voltage-gated type VIII alpha polypeptide splice variant voltage-gated sodium channel alpha subunit voltage-gated sodium channel subunit alpha Nav1.6 voltage-gated sodium channel type VIII alpha protein |
| Molecular Weight(MW) | 225kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q9UQD0 |
|---|
WB
Western blot analysis using Nav1.6/SCN8A antibody against Hela(1) ,A549(2),A431(3),Huvec(4),Nih3t3(5),Cos-7(6)cell . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
