WB
Western blot analysis of Nav1.6 / SCN8A on different lysates with Rabbit anti-Nav1.6 / SCN8A antibody at 1/2,000 dilution. Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Negative control : Liver.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Nav1.6 / SCN8A antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
Nav1.6 / SCN8A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input). Lane 2: Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody IP in mouse brain tissue lysate. Lane 3: Rabbit IgG instead of Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody in mouse brain tissue lysate. Blocking/Dilution buffer: primary antibody dilution. Exposure time: 1 minute 59 seconds.| Product Name | Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000-1:5000 IHC:1:500 IP:1-2μg/sample |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCN8A |
|---|---|
| Gene Synonyms | MED PN4 CIAT BFIS5 DEE13 NaCh6 CERIII EIEE13 MYOCL2 Nav1.6 |
| Gene Full Name | sodium voltage-gated channel alpha subunit 8 |
| Gene Summary | This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with cognitive disability, pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| Alternative Names | CerIII antibody CIAT antibody EIEE13 antibody hNa6/Scn8a voltage gated sodium channel antibody MED antibody Motor endplate disease antibody NaCh 6 antibody NaCh6 antibody Nav 1.6 antibody Nbna1 antibody peripheral nerve protein type 4 antibody PN 4 antibody PN4 antibody SCN8A antibody SCN8A_HUMAN antibody Sodium channel protein type 8 alpha subunit antibody Sodium channel protein type 8 subunit alpha antibody Sodium channel protein type VIII alpha subunit antibody Sodium channel protein type VIII subunit alpha antibody Sodium channel voltage gated type VIII alpha antibody Sodium channel voltage gated type VIII alpha polypeptide antibody Sodium channel voltage gated type VIII alpha subunit antibody Voltage gated sodium channel subunit alpha Nav1.6 antibody Voltage-gated sodium channel subunit alpha Nav1.6 antibody |
| Molecular Weight(MW) | 225 kDa(Observed band size: 260 kDa) |
| Cellular Localization | Cell membrane, Cell projection, axon; Cytoplasmic vesicle, Cell projection, podosome. |
WB
Western blot analysis of Nav1.6 / SCN8A on different lysates with Rabbit anti-Nav1.6 / SCN8A antibody at 1/2,000 dilution. Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Negative control : Liver.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Nav1.6 / SCN8A antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
Nav1.6 / SCN8A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input). Lane 2: Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody IP in mouse brain tissue lysate. Lane 3: Rabbit IgG instead of Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody in mouse brain tissue lysate. Blocking/Dilution buffer: primary antibody dilution. Exposure time: 1 minute 59 seconds.| Application Notes | WB:1:2000-1:5000 IHC:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
