ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.ICC/IF
Immunofluorescence analysis of NIH3T3 cells using CD182 antibody (green). Blue: DAPI fluorescent DNA dye.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160).| Product Name | Anti-CD182 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCR2 |
|---|---|
| Gene Synonyms | CD128 CD182 CDw128 CDw128b CMKAR2 Gpcr16 IL-8rb IL-8Rh IL8R2 IL8RA IL8RB mIL-8RH SCN13 WHIMS2 |
| Gene Full Name | C-X-C motif chemokine receptor 2 |
| Gene Summary | The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009] |
| Alternative Names | C Cmotif chemokine C X C motif chemokine CC motif chemokine CCmotif chemokine CD182 antigen chemokine (C-X-C motif) receptor 2 chemokine (C-X-C) receptor 2 chemokine (CXC) receptor 2 CXC CXC motif chemokine CXC-R2 CXCR-2 CXCR2 gene for IL8 receptor type B GRO/MGSA receptor high affinity interleukin-8 receptor B IL-8 receptor alpha chain Il-8 receptor b IL-8 receptor beta IL-8 receptor type 2 IL-8R B interleukin 8 receptor type 2 interleukin 8 receptor beta interleukin-8 receptor type B |
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P25025 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using CD182 antibody (green). Blue: DAPI fluorescent DNA dye.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160).| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
