Application
TUBB3 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).Application
TUBB3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).Application
TUBB3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TUBB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TUBB3 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TUBB3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TUBB4 |
|---|---|
| Alternative Names | TUBB3 TUBB4 Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III |
| Molecular Weight(MW) | 50433 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance |
| Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 10381 |
|---|---|
| Nucleotide Accession | NP_006077.2 |
Application
TUBB3 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
Application
TUBB3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
Application
TUBB3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TUBB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TUBB3 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM223909