Application
Western blot analysis of TUBB4 antibody (N-term) in A375 cell line lysates (35ug/lane). TUBB4 (arrow) was detected using the purified Pab.Application
Western blot analysis of TUBB4 antibody (N-term) in mouse brain tissue lysates (35ug/lane). TUBB4 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with TUBB4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TUBB4 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TUBB4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBB4A; TUBB4; TUBB5; Tubulin beta-4A chain; Tubulin 5 beta; Tubulin beta-4 chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TUBB4 TUBB5 |
|---|---|
| Alternative Names | TUBB4A TUBB4 TUBB5 Tubulin beta-4A chain Tubulin 5 beta Tubulin beta-4 chain |
| Molecular Weight(MW) | 49586 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain |
| Tissue Specificity | Major isotype in brain, where it represents 46% of all beta-tubulins |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 10382 |
|---|
Application
Western blot analysis of TUBB4 antibody (N-term) in A375 cell line lysates (35ug/lane). TUBB4 (arrow) was detected using the purified Pab.
Application
Western blot analysis of TUBB4 antibody (N-term) in mouse brain tissue lysates (35ug/lane). TUBB4 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with TUBB4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TUBB4 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM223914