Application
A. Western blot analysis on HeLa cell lysate using anti- ?-Tubulin, class III RabMAb (Cat. #AJ1098b), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Tubulin beta class III RabMAb (Cat. #AJ1098b)| Product Name | Beta-Tubulin-III Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-III;Tubulin beta-4 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1569Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50000~100000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TUBB4 |
|---|---|
| Alternative Names | TUBB3 TUBB4 Tubulin beta-3 chain Tubulin beta-III Tubulin beta-4 |
| Molecular Weight(MW) | 50433 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance |
| Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 10381 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti- ?-Tubulin, class III RabMAb (Cat. #AJ1098b), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Tubulin beta class III RabMAb (Cat. #AJ1098b)| Application Notes | WB:1:50000~100000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM227607