WB
Western blot analysis using PODXL antibody against Hek293(1), Hepg2(2),MCF-7(3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PODXL antibody with DAB staining.ICC/IF
Immunofluorescence analysis of NIH3T3 cells using PODXL antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-PODXL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PODXL |
|---|---|
| Gene Synonyms | PC PDX PCLP Gp200 gp135 PCLP-1 PODXL1 |
| Gene Full Name | podocalyxin like |
| Gene Summary | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 59KD |
| Cellular Localization | Cell membrane, Cell projection, Membrane |
WB
Western blot analysis using PODXL antibody against Hek293(1), Hepg2(2),MCF-7(3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PODXL antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using PODXL antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642545