Application
PC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PC Antibody for immunohistochemistry.Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC |
| Product description | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq].1) MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009) |
| Immunogen | This PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human PC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PC |
|---|---|
| Alternative Names | Pyruvate carboxylase mitochondrial Pyruvic carboxylase PCB PC |
| Molecular Weight(MW) | 130 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
PC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PC Antibody for immunohistochemistry.
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM287484