Immunoblot
1: 45ug human capan1 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: PCSubmitted by: Dr. Pankaj Kumar Singh, UNMC, Omaha, NEWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Live| Product Name | Rabbit anti-PC polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCB |
| Immunogen | The immunogen for anti-PC antibody: synthetic peptide directed towards the C terminal of human PC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanMousePig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PC |
|---|---|
| Gene Synonyms | PCB |
| Gene Full Name | Pyruvate carboxylase |
| Gene Summary | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. |
| Alternative Names | PCB |
| Molecular Weight(MW) | 127kDa |
| Sequence | 1178 amino acids |
| Entrez Gene | 5091 |
|---|---|
| SwissProt ID | P11498 |
| Protein Accession | NP_001035806 |
Immunoblot
1: 45ug human capan1 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: PCSubmitted by: Dr. Pankaj Kumar Singh, UNMC, Omaha, NE
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM118100