WB
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, All lanes: PODXL antibody at 2.5µg/ml, Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution.WB
Positive WB detected in: Mouse brain tissue, All lanes: PODXL antibody at 0.43µg/ml, Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution.WB
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue, All lanes: PODXL antibody at 1.28µg/ml, Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution.ICC/IF
Immunofluorescent analysis of Hela cells using PODXL Monoclonal Antibody at a dilution of 1:100 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).ICC/IF
Immunofluorescent analysis of PC-3 cells using PODXL Monoclonal Antibody at a dilution of 1:100 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using PODXL Monoclonal Antibody at dilution of 1:200.IHC
Immunohistochemistry of paraffin-embedded human melanoma using PODXL Monoclonal Antibody at dilution of 1:200.IP
Immunoprecipitating PODXL in HEK293 whole cell lysate. Lane 1: Mouse control IgG (1µg) instead of PODXL Monoclonal Antibody in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PODXL Monoclonal Antibody (8µg) + HEK293 whole cell lysate (500µg). Lane 3: HEK293 whole cell lysate (10µg).| Product Name | PODXL Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human PODXL protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:5000 IHC:1:50-1:500 ICC/IF:1:50-1:200 IP:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PODXL |
|---|---|
| Gene Synonyms | PC PDX PCLP Gp200 gp135 PCLP-1 PODXL1 |
| Gene Full Name | podocalyxin like |
| Gene Summary | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 59kDa |
| Cellular Localization | Apical cell membrane,Membrane. |
WB
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, All lanes: PODXL antibody at 2.5µg/ml, Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution.
WB
Positive WB detected in: Mouse brain tissue, All lanes: PODXL antibody at 0.43µg/ml, Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution.
WB
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue, All lanes: PODXL antibody at 1.28µg/ml, Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution.
ICC/IF
Immunofluorescent analysis of Hela cells using PODXL Monoclonal Antibody at a dilution of 1:100 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).
ICC/IF
Immunofluorescent analysis of PC-3 cells using PODXL Monoclonal Antibody at a dilution of 1:100 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).
IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using PODXL Monoclonal Antibody at dilution of 1:200.
IHC
Immunohistochemistry of paraffin-embedded human melanoma using PODXL Monoclonal Antibody at dilution of 1:200.
IP
Immunoprecipitating PODXL in HEK293 whole cell lysate. Lane 1: Mouse control IgG (1µg) instead of PODXL Monoclonal Antibody in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PODXL Monoclonal Antibody (8µg) + HEK293 whole cell lysate (500µg). Lane 3: HEK293 whole cell lysate (10µg).| Application Notes | WB:1:500-1:5000 IHC:1:50-1:500 ICC/IF:1:50-1:200 IP:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Data sheet for OM644250