Western blot
Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysatePrimary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRSWestern blot
1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-RAD17 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24, RAD17SP |
| Immunogen | The immunogen for anti-RAD17 antibody: synthetic peptide directed towards the C terminal of human RAD17 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAD17 |
|---|---|
| Gene Synonyms | CCYC HRAD17 R24L RAD17Sp Rad24 RAD24 RAD17SP |
| Gene Full Name | RAD17 homolog (S. pombe) |
| Gene Summary | RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. |
| Alternative Names | CCYC HRAD17 R24L RAD17Sp Rad24 RAD24 RAD17SP |
| Molecular Weight(MW) | 66kDa |
| Sequence | 584 amino acids |
Western blot
Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysatePrimary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS
Western blot
1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
