Western blot
Positive Control: Lane1: 25ug HeLa lysate, Lane2: 25ug Xenopus laevis egg extract, Lane3: 25ug mouse embryonic stem cell lysate, Lane4: 25ug HEK293T lysate Primary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRSWestern blot
1 ug/mlPositive Control: Fetal liver cell lysate| Product Name | Rabbit anti-RAD17 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCYC, FLJ41520, HRAD17, R24L, RAD17SP, RAD24 |
| Immunogen | The immunogen for anti-RAD17 antibody: synthetic peptide directed towards the N terminal of human RAD17 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAD17 |
|---|---|
| Gene Synonyms | CCYC FLJ41520 HRAD17 R24L RAD17SP RAD24 |
| Gene Full Name | RAD17 homolog (S. pombe) |
| Gene Summary | RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. |
| Alternative Names | CCYC FLJ41520 HRAD17 R24L RAD17SP RAD24 |
| Molecular Weight(MW) | 76kDa |
| Sequence | 670 amino acids |
Western blot
Positive Control: Lane1: 25ug HeLa lysate, Lane2: 25ug Xenopus laevis egg extract, Lane3: 25ug mouse embryonic stem cell lysate, Lane4: 25ug HEK293T lysate Primary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS
Western blot
1 ug/mlPositive Control: Fetal liver cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
