Western blot
Mouse stem cells expressed in cytoplasm and nucleoplasm. Primary antibody is 1:2000. 20ug lysate added to each lane. Lane 1: Mouse stem cell cytoplasm. Lane 2: Mouse stem cell nucleoplasm.| Product Name | Rabbit anti-CTCF Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for Anti-CTCF antibody is: synthetic peptide directed towards the C-terminal region of Human CTCF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTCF |
|---|---|
| Gene Synonyms | - |
| Gene Summary | This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. |
| Molecular Weight(MW) | 83kDa |
| Sequence | 727 amino acids |
Western blot
Mouse stem cells expressed in cytoplasm and nucleoplasm. Primary antibody is 1:2000. 20ug lysate added to each lane. Lane 1: Mouse stem cell cytoplasm. Lane 2: Mouse stem cell nucleoplasm.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
