Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.Application
Antibody used in CHIP on HCT116 Cells.| Product Name | CTCF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.1) Defossez, P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CTCF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CTCF antibody can be used for detection of CTCF by ELISA at 1:62500. CTCF antibody can be used for detection of CTCF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTCF |
|---|---|
| Alternative Names | CTCF MRD21 |
| Molecular Weight(MW) | 83 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in CHIP on HCT116 Cells.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | CTCF antibody can be used for detection of CTCF by ELISA at 1:62500. CTCF antibody can be used for detection of CTCF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CTCF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
