Immunohistochemical
Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution :1:300Secondary Antibody : Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution :1:200Color/Signal Descriptions :ZEB: Green DAPI: BlueGene Name :ZEB1Submitted by :Dr. Pankaj Singh, UNMC, Omaha, NEImmunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: ZEB1Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NE| Product Name | Rabbit anti-ZEB1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AREB6, BZP, DELTA-EF1, FECD6, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, PPCD3, DELTAEF1 |
| Immunogen | The immunogen for anti-ZEB1 antibody: synthetic peptide directed towards the N terminal of human ZEB1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZEB1 |
|---|---|
| Gene Synonyms | AREB6 BZP DELTA-EF1 FECD6 MGC133261 NIL-2-A NIL-2A NIL2A TCF8 ZEB ZFHEP ZFHX1A PPCD3 DELTAEF1 |
| Gene Full Name | Zinc finger E-box binding homeobox 1 |
| Gene Summary | ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. |
| Alternative Names | AREB6 BZP DELTA-EF1 FECD6 MGC133261 NIL-2-A NIL-2A NIL2A TCF8 ZEB ZFHEP ZFHX1A PPCD3 DELTAEF1 |
| Molecular Weight(MW) | 124kDa |
| Sequence | 1124 amino acids |
Immunohistochemical
Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution :1:300Secondary Antibody : Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution :1:200Color/Signal Descriptions :ZEB: Green DAPI: BlueGene Name :ZEB1Submitted by :Dr. Pankaj Singh, UNMC, Omaha, NE
Immunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: ZEB1Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NE| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
