Western blot
Titration: 1.0 ug/mlPositive Control: Fetal LungImmunoblot
1. 30 ug Cytosol extract: Untransfected cells 2. 30 ug Cytosol extract: Cells transfected with control (non-targeting) siRNA 3. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA 4. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNAPrimary Antibody Dilution: 1:2000Secondary Antibody: Goat anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: E2F1Submitted by: Ewelina Swiderek, Insitute of Immunology and Experimental Therapy, Wroclaw, Poland| Product Name | Rabbit anti-E2F1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F-1, RBAP1, RBBP3, RBP3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F1 |
|---|---|
| Gene Synonyms | E2F-1 RBAP1 RBBP3 RBP3 |
| Gene Full Name | E2F transcription factor 1 |
| Gene Summary | The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. |
| Alternative Names | E2F-1 RBAP1 RBBP3 RBP3 |
| Molecular Weight(MW) | 47kDa |
| Sequence | 437 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Lung
Immunoblot
1. 30 ug Cytosol extract: Untransfected cells 2. 30 ug Cytosol extract: Cells transfected with control (non-targeting) siRNA 3. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA 4. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNAPrimary Antibody Dilution: 1:2000Secondary Antibody: Goat anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: E2F1Submitted by: Ewelina Swiderek, Insitute of Immunology and Experimental Therapy, Wroclaw, Poland| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
