Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region and HCT116 CellsImmunohistochemistry
IHC Suggested Anti-HDAC2 antibody Titration: 5µg/ mlPositive Control: BreastWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysate| Product Name | Rabbit anti-HDAC2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPD3, YAF1, HD2 |
| Immunogen | The immunogen for anti-HDAC2 antibody: synthetic peptide directed towards the middle region of human HDAC2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC2 |
|---|---|
| Gene Synonyms | RPD3 YAF1 HD2 |
| Gene Full Name | Histone deacetylase 2 |
| Gene Summary | Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin struct |
| Alternative Names | RPD3 YAF1 HD2 |
| Molecular Weight(MW) | 65kDa |
| Sequence | 582 amino acids |
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region and HCT116 Cells
Immunohistochemistry
IHC Suggested Anti-HDAC2 antibody Titration: 5µg/ mlPositive Control: Breast
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
