Immunohistochemistry
Immunohistochemistry with mouse embryo tissue tissueImmunohistochemical
Sample Type: Frozen section of mouse embryo at et12.5Primary Dilution: 1:500Secondary Dilution: 1:2000Antigen Retrevial method: Citrate buffer (pH=6) Image Submitted By: Frédéric ClotmanUniversité catholique de LouvainWestern blot
Onecut1 antibody - C-terminal region validated by WB using Mouse Liver lysate at 1.0ug/ml.| Product Name | Rabbit anti-Onecut1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D9Ertd423e, Hfh12, Hnf6, Hnf6b, OC-1, Oc1, HNF-6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Onecut1 |
|---|---|
| Gene Synonyms | D9Ertd423e Hfh12 Hnf6 Hnf6b OC-1 Oc1 HNF-6 |
| Gene Full Name | One cut domain, family member 1 |
| Gene Summary | Onecut1 is a transcriptional activator. It binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. It is mportant for liver genes transcription and stimulates the expression of Onecut3 in the developing endoderm. |
| Alternative Names | D9Ertd423e Hfh12 Hnf6 Hnf6b OC-1 Oc1 HNF-6 |
| Molecular Weight(MW) | 51kDa |
| Sequence | 465 amino acids |
Immunohistochemistry
Immunohistochemistry with mouse embryo tissue tissue
Immunohistochemical
Sample Type: Frozen section of mouse embryo at et12.5Primary Dilution: 1:500Secondary Dilution: 1:2000Antigen Retrevial method: Citrate buffer (pH=6) Image Submitted By: Frédéric ClotmanUniversité catholique de Louvain
Western blot
Onecut1 antibody - C-terminal region validated by WB using Mouse Liver lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
