Immunohistochemistry
Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibodyWestern blot
Host: Rabbit Target Name:PAX7 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:0.0625ug/mL Peptide Concentration: 8.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%Immunoblot
1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: PAX7Submitted by: Anonymous| Product Name | Rabbit anti-PAX7 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HUP1, RMS2, PAX7B |
| Immunogen | The immunogen for anti-PAX7 antibody: synthetic peptide directed towards the N terminal of human PAX7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX7 |
|---|---|
| Gene Synonyms | HUP1 RMS2 PAX7B |
| Gene Full Name | Paired box 7 |
| Gene Summary | PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box gene 7 is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown. |
| Alternative Names | HUP1 RMS2 PAX7B |
| Molecular Weight(MW) | 57kDa |
| Sequence | 520 amino acids |
Immunohistochemistry
Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody
Western blot
Host: Rabbit Target Name:PAX7 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:0.0625ug/mL Peptide Concentration: 8.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%
Immunoblot
1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: PAX7Submitted by: Anonymous| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
