WB
Western blot analysis of extracts from Mouse brain, using PAX7 Antibody. The lane on the left was treated with blocking peptide.ICC/IF
PAX7 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to PAX7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PAX7(Accession P23759), corresponding to amino acid residues D178-Q228. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX7 |
|---|---|
| Gene Synonyms | HUP1 RMS2 PAX7B CMYO19 CMYP19 MYOSCO |
| Gene Full Name | paired box 7 |
| Gene Summary | This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
| Alternative Names | FLJ37460 HUP1 OTTHUMP00000002534 Paired box 7 Paired box gene 7 Paired box homeotic gene 7 Paired box protein Pax-7 Paired domain gene 7 Paired domain gene HuP1 Pax7 PAX7 transcriptional factor PAX7/FKHR fusion gene included PAX7_HUMAN PAX7B RGD1564360 RMS2 |
| Molecular Weight(MW) | 57kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts from Mouse brain, using PAX7 Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
PAX7 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
