Application
WB Suggested Anti-NFKBIB Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:2500Positive Control: Jurkat cell lysateNFKBIB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cellsApplication
Lanes: Lane 1: 100ug mouse liver lysate Lane 2: 100ug mouse brain lysate Lane 3: 100ug mouse heart lysate Lane 4: 100ug mouse kidney lysate Lane 5: 100ug mouse lung lysate Lane 6: 100ug mouse thymus lysate Lane 7: 100ug mouse spleen lysate Lane 8: 100ug mouse testis lysate Lane 9: 100ug HeLa cell lysate Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-APSecondary Antibody Dilution: 1:10,000Gene Name: NFKBIBSubmitted by: Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication
Host: RabbitTarget Name: NFKBIBSample Tissue: Mouse LiverAntibody Dilution: 1ug/ml| Product Name | Rabbit anti-NFKBIB polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKBB, TRIP9 |
| Immunogen | The immunogen for anti-NFKBIB antibody: synthetic peptide directed towards the N terminal of human NFKBIB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIB |
|---|---|
| Gene Synonyms | NFKBIB |
| Gene Full Name | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta |
| Gene Summary | NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. |
| Alternative Names | IKBB TRIP9 |
| Molecular Weight(MW) | 38kDa |
| Sequence | 356 amino acids |
Application
WB Suggested Anti-NFKBIB Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:2500Positive Control: Jurkat cell lysateNFKBIB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Application
Lanes: Lane 1: 100ug mouse liver lysate Lane 2: 100ug mouse brain lysate Lane 3: 100ug mouse heart lysate Lane 4: 100ug mouse kidney lysate Lane 5: 100ug mouse lung lysate Lane 6: 100ug mouse thymus lysate Lane 7: 100ug mouse spleen lysate Lane 8: 100ug mouse testis lysate Lane 9: 100ug HeLa cell lysate Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-APSecondary Antibody Dilution: 1:10,000Gene Name: NFKBIBSubmitted by: Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) Group
Application
Host: RabbitTarget Name: NFKBIBSample Tissue: Mouse LiverAntibody Dilution: 1ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
