Application
WB Suggested Anti-ZYX Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human StomachApplication
WB Suggested Anti-ZYX antibody Titration: 1 ug/mLSample Type: Human heartApplication
Rabbit Anti-ZYX Antibody Catalog Number: Formalin Fixed Paraffin Embedded Tissue: Human Adult liver Observed Staining: CytoplasmicPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 – 2.0 secProtocol located in Reviews and Data.| Product Name | Rabbit anti-ZYX polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESP-2, HED-2 |
| Immunogen | The immunogen for anti-ZYX antibody: synthetic peptide directed towards the middle region of human ZYX |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZYX |
|---|---|
| Gene Synonyms | ZYX |
| Gene Full Name | Zyxin |
| Gene Summary | TRIM50 is an E3 ubiquitin-protein ligase.Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. |
| Alternative Names | ESP-2 HED-2 |
| Molecular Weight(MW) | 61kDa |
| Sequence | 572 amino acids |
Application
WB Suggested Anti-ZYX Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human Stomach
Application
WB Suggested Anti-ZYX antibody Titration: 1 ug/mLSample Type: Human heart
Application
Rabbit Anti-ZYX Antibody Catalog Number: Formalin Fixed Paraffin Embedded Tissue: Human Adult liver Observed Staining: CytoplasmicPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 – 2.0 secProtocol located in Reviews and Data.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
