Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.Application
Antibody used in WB on Human HeLa cells at 1:200.| Product Name | ZYX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZYX, ESP-2, HED-2 |
| Product description | Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.1) Li, B., et al., (2004) J. Biol. Chem. 279 (19), 20401-20410. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZYX. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZYX antibody can be used for detection of ZYX by ELISA at 1:62500. ZYX antibody can be used for detection of ZYX by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZYX |
|---|---|
| Alternative Names | ZYX ESP-2 HED-2 |
| Molecular Weight(MW) | 61 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Application
Antibody used in WB on Human HeLa cells at 1:200.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ZYX antibody can be used for detection of ZYX by ELISA at 1:62500. ZYX antibody can be used for detection of ZYX by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZYX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
