Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human SpleenWestern blot
YBX1 antibody - middle region validated by WB using 1. Human NT-2 cells (60ug) at 2ug/ml.Western blot
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: AnonymousImmunohistochemical
Placenta| Product Name | Rabbit anti-YBX1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BP-8, CSDA2, CSDB, DBPB, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP-1, NSEP1, YB-1, YB1 |
| Immunogen | The immunogen for anti-YBX1 antibody: synthetic peptide directed towards the middle region of human YBX1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YBX1 |
|---|---|
| Gene Synonyms | BP-8 CSDA2 CSDB DBPB MDR-NF1 MGC104858 MGC110976 MGC117250 NSEP-1 NSEP1 YB-1 YB1 |
| Gene Full Name | Y box binding protein 1 |
| Gene Summary | YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. YBX1 binds and stabilizes cytoplasmic mRNA. YBX1 contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. YBX1 binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. YBX1 regulates the transcription of numerous genes. YBX1 promotes separation of DNA strands that contain mismatches or are modified by cisplatin. YBX1 has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). YBX1 may play a role in DNA repair. YBX1 is the component of the CRD-mediated complex that promotes MYC mRNA stability. |
| Alternative Names | BP-8 CSDA2 CSDB DBPB MDR-NF1 MGC104858 MGC110976 MGC117250 NSEP-1 NSEP1 YB-1 YB1 |
| Molecular Weight(MW) | 36kDa |
| Sequence | 324 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Spleen
Western blot
YBX1 antibody - middle region validated by WB using 1. Human NT-2 cells (60ug) at 2ug/ml.
Western blot
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: Anonymous
Immunohistochemical
Placenta| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
