Immunohistochemical
ImmunohistochemicalWestern blot
1.25ug/mlPositive Control: Transfected 293T| Product Name | Rabbit anti-TAF6 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp781E21155, MGC:8964, TAF2E, TAFII70, TAFII80, TAFII85, TAFII-70, TAFII-80, TAF(II)70, TAF(II)80 |
| Immunogen | The immunogen for anti-TAF6 antibody: synthetic peptide directed towards the N terminal of human TAF6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TAF6 |
|---|---|
| Gene Synonyms | DKFZp781E21155 MGC:8964 TAF2E TAFII70 TAFII80 TAFII85 TAFII-70 TAFII-80 TAF(II)70 TAF(II)80 |
| Gene Full Name | TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa |
| Gene Summary | TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1,the largest subunit of TFIID. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. |
| Alternative Names | DKFZp781E21155 MGC:8964 TAF2E TAFII70 TAFII80 TAFII85 TAFII-70 TAFII-80 TAF(II)70 TAF(II)80 |
| Molecular Weight(MW) | 71kDa |
| Sequence | 667 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
1.25ug/mlPositive Control: Transfected 293T| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
