Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Product Name | TAF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAF6, DKFZp781E21155, MGC:8964, TAF2E, TAFII70, TAFII80, TAFII85, TAFII-70, TAFII-80, TAF(II)70, TAF(II)80 |
| Product description | TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1,the largest subunit of TFIID. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.1) Kimura, K., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| TAF6 antibody can be used for detection of TAF6 by ELISA at 1:1562500. TAF6 antibody can be used for detection of TAF6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TAF6 |
|---|---|
| Alternative Names | TAF6 DKFZp781E21155 MGC:8964 TAF2E TAFII70 TAFII80 TAFII85 TAFII-70 TAFII-80 TAF(II)70 TAF(II)80 |
| Molecular Weight(MW) | 71 kDa, 73 kDa, 71 kDa, 73 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | TAF6 antibody can be used for detection of TAF6 by ELISA at 1:1562500. TAF6 antibody can be used for detection of TAF6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TAF6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
