Western blot
Positive Control: Lane 1: 60ug HCT116 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:5,000Submitted by: Chinweike Ukomadu, Brigham and Women's Hospital, BostonWestern blot
Titration: 1.0 ug/mlPositive Control: Mouse Pancreas| Product Name | Rabbit anti-Uhrf2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2310065A22Rik, AI426270, AW214556, D130071B19Rik, Nirf |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Uhrf2 |
|---|---|
| Gene Synonyms | 2310065A22Rik AI426270 AW214556 D130071B19Rik Nirf |
| Gene Full Name | Ubiquitin-like, containing PHD and RING finger domains 2 |
| Gene Summary | Uhrf2 is an E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of PCNP. It may participate in methylation-dependent transcriptional regulation. It is important for G1/S transition. Overexpression causes G1 phase cell arrest. |
| Alternative Names | 2310065A22Rik AI426270 AW214556 D130071B19Rik Nirf |
| Molecular Weight(MW) | 90kDa |
| Sequence | 803 amino acids |
Western blot
Positive Control: Lane 1: 60ug HCT116 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:5,000Submitted by: Chinweike Ukomadu, Brigham and Women's Hospital, Boston
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Pancreas| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
