Immunoblot
Lane 1: 30ug mouse renal epithelial lysateLane 2: 30ug mouse renal epithelial lysateLane 3: 30ug mouse renal epithelial lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:2500Gene Name: CLIC1Submitted by: Dr. JUNMING FAN,ECUWestern blot
Sample Type: Purified Recombinant CLIC-1 proteinPrimary Dilution: 1:1000Western blot
CLIC1 antibody - C-terminal region validated by WB using purified recombinant CLIC1 protein and transfected lysate| Product Name | Rabbit anti-CLIC1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G6, NCC27 |
| Immunogen | The immunogen for anti-CLIC1 antibody: synthetic peptide directed towards the C terminal of human CLIC1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGoatHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CLIC1 |
|---|---|
| Gene Synonyms | G6 NCC27 |
| Gene Full Name | Chloride intracellular channel 1 |
| Gene Summary | Chloride intracellular channel 1 is a member of the p64 family, a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIon Channel1 encodes a protein that localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. |
| Alternative Names | G6 NCC27 |
| Molecular Weight(MW) | 27kDa |
| Sequence | 241 amino acids |
Immunoblot
Lane 1: 30ug mouse renal epithelial lysateLane 2: 30ug mouse renal epithelial lysateLane 3: 30ug mouse renal epithelial lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:2500Gene Name: CLIC1Submitted by: Dr. JUNMING FAN,ECU
Western blot
Sample Type: Purified Recombinant CLIC-1 proteinPrimary Dilution: 1:1000
Western blot
CLIC1 antibody - C-terminal region validated by WB using purified recombinant CLIC1 protein and transfected lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
